TheAfricanturquoisekillifishNothobranchiusfurzeriisashort-livedfishspeciesthathasbeenestablishedasanovelvertebratemodeltostudyagingandrepresentsapromisingsystemtoexaminevariousotherprocesses.Thekillifishisrelativelyeasyandinexpensivetomaintain.Inaddition,ithasashortlifecycleandabundantprogeny,facilitatingitsuse.Investigationusingthismodelorganismisgrowingandhasprovidednewinsightsintoaging,age-relateddiseases,ecology,evolution,andembryonicdiapause--astateof“suspendedanimation.“ThislaboratorymanualprovidesacomprehensivecollectionofprotocolscoveringexperimentaltechniquescurrentlyusedtostudytheAfricanturquoisekillifish.Husbandryprotocolsdescribehowtosetupandmaintainakillifishcolonyandhowtogenerateandpreservegeneticallyengineeredkillifishlines.Thiscollectionalsoincludesbothgeneralandspecificmethodstocharacterizedifferentorgansduringaging,withaparticularfocusonthebrain.Severalgenomicsmethodsandanalysesforunbiasedcharacterizationarealsodescribed.Finally,thismanualencompassesprotocolsinotherinterestingareasofresearch,includinginjuryrepair,development,andembryonicdiapause.ThiscollectionshouldenablestudiesintheAfricanturquoisekillifishandotherspeciesofkillifishsuchastheSouthAmericankillifish,andincludesprotocolsthatwillbeusefulbothtogroupsconsideringtheuseofthesesystemsforthefirsttimeandtothosewithexperienceinthefield.Wealsohopethatthisprotocolbookwillfosteralargekillifishcommunityandwillpromotenewstudiesinmanydifferentresearchareas.
